Techniques for sequencing and mapping. Gene content in Archaea, Bacteria and Eukaryotes. Comparative genomics. Gene evolution and gene families. Principles of functional genomics. Comparative trascriptomics. Genome variability. Statistical analysis of genome variability. Environmental genomics: discoveries and applications. Bioinformatic tools for the study of structure and function of genomes
Textbooks:
A.M. Lesk, Introduction to genomics. Oxford University Press, 2008
B. Lewin, Genes IX, Jones and Bartlett, 2008
S.B. Primrose & R.M. Twyman, Principles of genome analysis and genomics. Blackwell Publishing, 2003
A. Scialpi & A. Mengoni (a cura di). La PCR e le sue varianti. Firenze University Press, 2008
Learning Objectives
Knowledge acquired: Basic and advanced knowledge on structural, comparative and functional genomics. Knowledge on methodologies for genome analysis
Competence acquired
The student has acquired competence on the study of genomes and on the experimental and bioinformatic techniques for their investigation
Skills acquired (at the end of the course):
The student is able to autonomously set up an experimental and bioinformatic protocol for genome analysis and see the outcomes in both biological and biotechnological terms.
Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other individual formative activities: 102
Contact hours for: Lectures (hours): 48
Contact hours for: Laboratory (hours):
Contact hours for: Laboratory-field/practice (hours): 0
Seminars (hours): 0
Stages: 0
Intermediate examinations: 0
Further information
Office hours:
Upon request from Monday to Friday
Type of Assessment
Exam modality:
Oral
Course program
Introduction to genomics. The history of genome sequencing. Techniques for sequencing and mapping. Gene content in Archaea, Bacteria and Eukaryotes. Repeated DNA. Nuclear and organelles genomes. Comparative genomics and the evolution of cells. Gene evolution and gene families. Isocores. Evolution of genome architecture. Model organisms and their genomes. Principles of functional genomics. Microarrays and the study of gene expression. The concept of gene and functional genomics. Control of gene expression at the whole genome level. Comparative trascriptomics. Genome variability: identification, origin and methodologies. QTL, molecular markers and population genomics. Statistical analysis of genome variability. Environmental genomics: discoveries and applications. Bioinformatic tools for the study of structure and function of genomes